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Molecular probe for identification of Trichomonas vaginalis DNA

Rubino, Salvatore and Muresu, Rosella and Rappelli, Paola and Fiori, Pier Luigi and Rizzu, Patrizia and Erre, Giuseppe and Cappuccinelli, Pietro Antonio (1991) Molecular probe for identification of Trichomonas vaginalis DNA. Journal of Clinical Microbiology, Vol. 29 (4), p. 702-706. eISSN 1098-660X. Article.

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Abstract

Trichomoniasis is one of the most widespread sexually transmitted diseases in the world. Diagnosis can be achieved by several methods, such as direct microscopic observation of vaginal discharge, cell culture, and immunological techniques. A 2.3-kb Trichomonas vaginalis DNA fragment present in strains from diverse geographic areas was cloned and used as a probe to detect T. vaginalis DNA in vaginal discharge by a dot blot hybridization technique. This probe was specific for T. vaginalis DNA. It recognized strains from two regions in Italy (Sardinia, Piemonte) and from Mozambique (Africa). In addition, our probe did not cross-react with bacterial (Escherichia coli, Enterococcus spp., group B streptococci, Gardnerella vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, and Lactobacillus spp.), viral (herpes simplex virus type 2), fungal (Candida albicans), protozoan (Entamoeba histolytica, Giardia lamblia, Plasmodium falciparum, Leishmania major, and Leishmania infantum), or human nucleic acids. The probe reacted with Pentatrichomonas hominis and Trichomonas foetus. The limit signal recognized by our probe corresponded to the DNA of 200 T. vaginalis isolates. The 2.3-kb probe was used in a clinical analysis of 98 samples. Of these, 20 samples were found to be positive both with the probe and by cell culture, and only 14 of these were positive by a standard wet mount method.

Item Type:Article
ID Code:7917
Status:Published
Refereed:Yes
Uncontrolled Keywords:Trichomoniasis, molecular probe, Pentatrichomonas hominis, Trichomonas foetus
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto per il sistema produzione animale in ambiente Mediterraneo, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-660X
Deposited On:30 Aug 2012 09:40

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