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Sequence of cDNA coding for a 65 kDa adhesive protein for the specific detection of Trichomonas vaginalis by PCR

Rappelli, Paola and Rocchigiani, Angela Maria and Erre, Giuseppe and Colombo, Mauro Maria and Cappuccinelli, Pietro Antonio and Fiori, Pier Luigi (1995) Sequence of cDNA coding for a 65 kDa adhesive protein for the specific detection of Trichomonas vaginalis by PCR. FEMS Microbiology Letters, Vol. 129 (1), p. 21-26. eISSN 1574-6968. Article.

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Abstract

A Trichomonas vaginalis cDNA library was constructed and recombinant plaques were screened using rabbit immunoglobulins specific for P65, a protozoan protein involved in pathogenicity that we identified in a previous study. A 1.38 kilobases cDNA fragment coding for the P65 protein was cloned in E. coli and then sequenced. On the basis of of the sequence obtained, six primers were synthesised and used to set up a Polymerase Chain Reaction. The presence of a specific amplicon in all 30 clinical isolates tested shows that P65 is a conserved and stable gene. The reaction is highly sensitive (as few as 5 to 10 parasites can be detected) and specific for Trichomonas vaginalis; the gene coding P65 adhesin can be therefore considered a very good molecular target for polymerase chain reaction-based diagnostic purposes.

Item Type:Article
ID Code:7913
Status:Published
Refereed:Yes
Uncontrolled Keywords:Trichomonas vaginalis, cDNA, P65, Polymerase Chain Reaction
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Blackwell / Wiley
eISSN:1574-6968
Deposited On:29 Aug 2012 17:00

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