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Development of a nested PCR for detection of Cryptococcus neoformans in cerebrospinal fluid

Rappelli, Paola and Are, Riccardo and Casu, Giuseppe and Fiori, Pier Luigi and Cappuccinelli, Pietro Antonio and Aceti, Antonio (1998) Development of a nested PCR for detection of Cryptococcus neoformans in cerebrospinal fluid. Journal of Clinical Microbiology, Vol. 36 (11), p. 3438-3440. eISSN 1098-660X. Article.

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Abstract

We report the development of a nested-PCR-based assay for the detection of Cryptococcus neoformans in cerebrospinal fluid. The specificity and sensitivity of the test were assessed. The technique was then applied to 40 cerebrospinal fluid samples. We obtained positive reactions for all 21 clinical samples from patients who had been previously diagnosed as having cryptococcal meningitis by conventional techniques and negative reactions for all 19 negative controls. Nested PCR is here compared with other diagnostic methods currently used in patients’ follow-up exams during anticryptococcal therapy.

Item Type:Article
ID Code:7908
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cryptococcus neoformans, cerebrospinal fluid, nested PCR
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-660X
Deposited On:29 Aug 2012 13:00

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