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A Pre-existing infection by Mycobacterium avium subsp. avium modulates anti-Cryptococcus neoformans and anti-Candida albicans activities in human macrophages

Fiori, Pier Luigi and Rappelli, Paola and Casu, Giuseppe and Delogu, Giuseppe and Turrini, Francesco Michelangelo and Cappuccinelli, Pietro Antonio (2000) A Pre-existing infection by Mycobacterium avium subsp. avium modulates anti-Cryptococcus neoformans and anti-Candida albicans activities in human macrophages. Microbial Pathogenesis, Vol. 29 (2), p. 93-100. eISSN 1096-1208. Article.

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DOI: 10.1006/mpat.2000.0368

Abstract

Mycobacterium avium is a facultative intracellular microorganism, able to survive and multiply within mammalian macrophages by circumventing antimicrobial mechanisms. In this study we hypothesize that pre-existing M. avium infection could result in macrophage superinfections by other microorganisms. We found that 24 h after ingestion of M. avium at a low multiplicity of infection, macrophages are unable to efficiently produce superoxide anions when over-stimulated with phorbol esters, and that the generation of oxidative burst is only partially restored 72 h after bacteria ingestion. We also demonstrate that intracellular killing of Cryptococcus neoformans is markedly impaired in human macrophages that have previously ingested M. avium (but not other bacteria such as Escherichia coli). This inhibitory effect is observed with live mycobacteria, but not when heat-inactivated bacteria are ingested. In contrast, whenCandida albicans is given to macrophages instead of C. neoformans, an enhancement of intracellular killing is observed, suggesting that cytocidal mechanisms other than respiratory burst are involved in the anti- Candidacidal activity of macrophages.

Item Type:Article
ID Code:7900
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium avium, intracellular killing, coinfection, macrophage, Cryptococcus neoformans, Candida albicans
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Academic Press
eISSN:1096-1208
Deposited On:29 Aug 2012 08:51

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