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Cloning and molecular characterization of a cDNA clone coding for Trichomonas vaginalis alpha-actinin and intracellular localization of the protein

Addis, Maria Filippa and Rappelli, Paola and Delogu, Giuseppe and Carta, Franco and Cappuccinelli, Pietro Antonio and Fiori, Pier Luigi (1998) Cloning and molecular characterization of a cDNA clone coding for Trichomonas vaginalis alpha-actinin and intracellular localization of the protein. Infection and Immunity, Vol. 66 (10), p. 4924-4931. eISSN 1098-5522. Article.

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Abstract

We have identified and sequenced a cDNA clone coding for Trichomonas vaginalis alpha-actinin. Analysis of the obtained sequence revealed that the 2,857-nucleotide-long cDNA contained an open reading frame encoding 849 amino acids which showed consistent homology with alpha-actinins of different species. Such homology was particularly significant in regions which have been reported to represent the actin-binding and Ca2+-binding domains in other alpha-actinins. The deduced protein was also characterized by the presence of a divergent central region thought to play a role in its high immunogenicity. A study of protein localization performed by immunofluorescence revealed that the protein is diffusely distributed throughout the T. vaginalis cytoplasm when the cell is pear shaped. When parasites adhere and transform into the amoeboid morphology, the protein is located only in areas close to the cytoplasmic membrane and colocalizes with actin. Concomitantly with transformation into the amoeboid morphology, alpha-actinin mRNA expression is upregulated.

Item Type:Article
ID Code:7898
Status:Published
Refereed:Yes
Uncontrolled Keywords:Trichomonas vaginalis, cDNA clone, molecular characterization
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-5522
Deposited On:28 Aug 2012 13:36

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