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By releasing ADP, Acanthamoeba castellanii causes an increase in the cytosolic free calcium concentration and apoptosis in wish cells

Mattana, Antonella and Tozzi, Maria Grazia and Costa, Maria Luisa and Delogu, Giuseppe and Fiori, Pier Luigi and Cappuccinelli, Pietro Antonio (2001) By releasing ADP, Acanthamoeba castellanii causes an increase in the cytosolic free calcium concentration and apoptosis in wish cells. Infection and Immunity, Vol. 69 (6), p. 4134-4140. eISSN 1098-5522. Article.

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DOI: 10.1128/IAI.69.6.4134-4140.2001

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Abstract

The role played by soluble molecules that may participate in acanthamoebal cytopathogenicity has yet to be fully characterized. We demonstrate here that Acanthamoeba castellanii trophozoites constitutively release ADP in the medium. Cell-free supernatants prepared from A. castellanii, by interaction with specific P2y2 purinoceptors expressed on the Wish cell membrane, caused a biphasic rise in [Ca2+]i, extensive cell membrane blebbing, cytoskeletal disorganization, and the breakdown of nuclei. Cell damage induced by amoebic supernatants was blocked by the P2y2 inhibitor Suramin. The same results were found in Wish cells exposed to purified ADP. These findings suggest that pathogenic free-living A. castellanii may have a cytopathic effect on human epithelial cells through ADP release, by a process that begins with a rise of cytosolic free-calcium concentration, and culminates in apoptosis.

Item Type:Article
ID Code:7896
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cytoskeleton, nucleotides, apoptosis, cytosolic free-calcium, Acanthamoeba
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:American Society for Microbiology
eISSN:1098-5522
Deposited On:28 Aug 2012 12:01

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