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Influence of culture media on the motility and viability of cryopreserved stallion spermatozoa

Leoni, Giovanni Giuseppe and Rosati, Irma and Berlinguer, Fiammetta and Ledda, Sergio and Pintus, Pier Paolo and Bogliolo, Luisa and Cherchi, Raffaele and Naitana, Salvatore (2002) Influence of culture media on the motility and viability of cryopreserved stallion spermatozoa. Reproduction in Domestic Animals, Vol. 37 (4), p. 241. eISSN 0936-6768. Article.

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DOI: 10.1046/j.1439-0531.2002.00380.x

Abstract

Thawed cryopreserved stallion semen was washed in PBS+0.3%BSA at 900 g for 5¢and cultured in 35 mm petri dishes in two different media: SOF+0.3%BSA (SOF) and TCM199+0.3% BSA (TCM) supplemented or not with 1500 UI/ml SOD. At 0, 3, 6, 9, 24 and 48 h of culture motility, viability and acrosome status were evaluated with propidium iodide and FITC-PSA. Results indicate that mass motility, measured as a 0–10 grade scale by three different operators, was higher in SOF than in TCM (7.3 vs. 3.7, 6 vs. 3, 4.3 vs. 2.3, 2 vs. 1, 0.7 vs. 0 at 3, 6, 9, 12, 24 and 48 h of culture respectively). SOD supplementation increased spermatozoa motility compared to the basic media (SOF: 8.7, 8.3, 6.3, 3, 2.39; TCM: 5.3, 4, 2.7,1, 0 for the same times). Cell viability was higher (p < 0:01) in SOF compared to TCM (3h:50.0 and 45, 6h : 54.8% vs. 32.3%, 9h : 39.1% vs. 15.5%, 24h : 14.5% vs. 2.0%, 48h : 5.5% vs. 0 for SOF and TCM, respectively). The viability rate was increased (p < 0:05) in TCM+SOD (3h : 45.1 vs. 54.1, 6h : 32.3 vs. 44.1, 9h : 15.5 vs. 37.5 and 24h : 2 vs. 10) but not in SOF+SOD. The rate of live spermatozoa with intact acrosome membrane was 52.5% at thawing and decreased significantly during the first 3 h of culture in both media (17.1% and 15.4% for SOD and TCM respectively, p < 0:01). SOD supplementation to both media did not influence acrosome integrity during the culture. SOF appeared as more efficient than TCM to preserve stallion sperm viability and motility during in vitro culture.

Item Type:Article
ID Code:7831
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cryopreserved stallion semen, viability, motility
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto incremento ippico per la Sardegna, Ozieri
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
eISSN:0936-6768
Additional Information:Poster presented at the 6. Annual conference of the European Society for Domestic Animal Reproduction (ESDAR), Parma, 12-14 September 2002.
Deposited On:25 Jul 2012 13:58

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