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Developmental competence of sheep oocytes collected by OPU after different ovarian hyperstimulation treatments

Berlinguer, Fiammetta and Rosati, Irma and Bogliolo, Luisa and Ledda, Sergio and Pintus, Pier Paolo and Leoni, Giovanni Giuseppe and Naitana, Salvatore (2002) Developmental competence of sheep oocytes collected by OPU after different ovarian hyperstimulation treatments. Reproduction in Domestic Animals, Vol. 37 (4), p. 226. eISSN 0936-6768. Article.

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DOI: 10.1046/j.1439-0531.2002.00380.x

Abstract

Our aim was to compare the developmental capacity of sheep oocytes obtained by OPU and then matured, fertilised and cultured in vitro after two different ovarian hyperstimulation treatments. Sheep were divided into three groups: a) constant FSH doses administration; b) decreasing FSH doses administration; c) no FSH treatments. Sheep were synchronised by the insertion of intravaginal sponges left in situ during 11 days; FSH (total dose: 96 I.U.) was administered in 4 doses given every 12 hours starting on day 9. Twelve hours after the last FSH administration sheep were restrained on a laparoscopic table under general anaesthesia and follicles were aspirated utilising an 18 G needle. In group a, we put in culture for the successive in vitro procedures (IVM/IVF/IVC) 86.2% of the oocytes recovered (14.5 oocytes/OPU), obtained 28.3% blastocysts. In group b, we cultured 91.3% of the oocytes recovered (17.4 oocytes/OPU), obtained 16% blastocysts. In group c, we cultured 76.2% of the oocytes recovered (10.6 oocytes/OPU), obtaining 7.3% blastocysts. These results indicate that there is a significant difference (p < 0:05) between the numbers of embryos obtained in treated versus non treated animals, while between the two ovarian hyperstimulation treatments we found no significant difference. (Supported by RAIZ).

Item Type:Article
ID Code:7830
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sheep oocytes, OPU, hyperstimulation treatments
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
eISSN:0936-6768
Additional Information:Poster presented at the 6. Annual conference of the European Society for Domestic Animal Reproduction (ESDAR), Parma, 12-14 September 2002.
Deposited On:25 Jul 2012 13:44

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