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Comparison between ejaculated and epididymal stallion sperm cryopreserved with the pellet method

Rosati, Irma and Berlinguer, Fiammetta and Ledda, Sergio and Bogliolo, Luisa and Cherchi, Raffaele and Leoni, Giovanni Giuseppe and Naitana, Salvatore (2002) Comparison between ejaculated and epididymal stallion sperm cryopreserved with the pellet method. Reproduction in Domestic Animals, Vol. 37 (4), p. 250. eISSN 0936-6768. Article.

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DOI: 10.1046/j.1439-0531.2002.00380.x

Abstract

In order to understand the freezability of epididymal sperm and its fertilising potential we compared the membrane integrity and the capacity to bind to the zona pellucida of frozen/thawed epididymal and ejaculated sperm. Epididymal semen (EpS) was harvested by flushing the epididymal tails of the testis obtained by standing castrations of 3-year old colts while ejaculated semen (EjS) was collected by artificial vagina. Semen was cryopreserved with the pellet method. Sperm viability and acrosome integrity were evaluated after thawing by incubating spermatozoa with propidium iodide and FITCPSA. Fertilising potential was assessed by glycerol-Hoechst staining of IVM horse and ovine oocytes coincubated with spermatozoa for 24 h. After thawing both EpS and EjS presented 50% viability, but a significant difference (Chi-Square test) was evidenced in the percentage of live acrosome reacted spermatozoa (EpS: 6%; EjS: 36.1%; p < 0:001). Zona binding test both with horse (EpS: 0.5±0.5; EjS: 0.3±0.5; p=0.285) and ovine (EpS: 5±7.5; EjS: 3.6±5.6; p=0.246) oocytes did not revealed any significant difference (ANOVA) between EpS and EjS. The results suggest that epididymal stallion spermatozoa can be cryopreserved successfully with a better membrane integrity and an equal capacity to bind to the zona pellucida in vitro compared to ejaculated spermatozoa.

Item Type:Article
ID Code:7829
Status:Published
Refereed:Yes
Uncontrolled Keywords:Epididymal semen (EpS), ejaculated semen (EjS), fertilising potential
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto incremento ippico per la Sardegna, Ozieri
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
eISSN:0936-6768
Additional Information:Poster presented at the 6. Annual conference of the European Society for Domestic Animal Reproduction (ESDAR), Parma, 12-14 September 2002.
Deposited On:25 Jul 2012 13:20

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