Juliano, Claudia Clelia Assunta and Galleri, Grazia and Klemetsrud, Therese and Karlesen, Jan and Giunchedi, Paolo (2011) Effect of chitosan malate on viability and cytoskeletal structures morphology of Caco-2 cells. International Journal of Pharmaceutics, Vol. 420 (2), p. 223-230. ISSN 0378-5173. eISSN 1873-3476. Article.
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The aim of this work was to evaluate the effects of the treatment with chitosan malate (CM) on viability of Caco-2 cells and on the morphology and the integrity of their cytoskeletal structures (microtubules, microfilaments). Cytotoxicity of CM, both as a solution and as microparticles obtained by spray drying, was evaluated by using the reduction of MTT reagent; microtubule and microfilaments organization of Caco-2 cells treated with CM solution was examined with immunofluorescence techniques in monolayers fixed with the glutaraldehyde–borohydride method.
CM as a solution displayed a concentration-dependent cytotoxicity towards Caco-2 cells, with viability
percentages of 5 ± 2%, 7 ± 3% and 31 ± 15% at 15, 10 and 5 mg/mL, respectively, while at 2.5 mg/mL or less cell viability was 90% or higher. CM microparticles also produced a remarkable cytotoxic effect (cell viability 84
± 17%, 16 ± 8% and 5 ± 6% after treatment with 1, 5 and 10 mg CM per well, respectively), resulting more toxic than CM solution.
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