Cappuccinelli, Pietro Antonio and Fiori, Pier Luigi and Gargani, G. and Pacetti, A.M. (1986) Antigenic differences between Brucella abortus and Brucella melitensis recognized by a monoclonal antibody. Microbiologica, Vol. 9 (2), p. 179-188. ISSN 0391-5352. Article.
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By means of somatic hybridization of spleen cells from BALB/c mice inoculated with Brucella abortus and of a line of murine myeloma, a monoclone was obtained producing an antibody which specifically recognizes an epitope of Brucella abortus by means of the agglutination reaction. This epitope is not only on the surface of the homologous strains but on all the Brucella abortus biotype I, on the Brucella melitensis biotype II and on the Brucella suis biotype II tested. On the contrary it is not present on the Brucella melitensis biotype I and in just one of six Brucella melitensis biotype III. Very few strains of the other biotypes of Brucella suis were tested: the results are often negative.
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