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Localization of beta-endorphins in the rat spinal cord using avidin-biotin technology

De Riu, Pier Luigi and Bitti, A. and Rubino, Salvatore and Virdis, G. and Fiori, Pier Luigi (1986) Localization of beta-endorphins in the rat spinal cord using avidin-biotin technology. Basic and Applied Histochemistry, Vol. 30 (4), p. 495-499. ISSN 0391-7258. Article.

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Abstract

The avidin-biotin-peroxidase technique is proposed for the immunological localization of beta-endorphin in the rat spinal cord. A rabbit specific antibody anti-human beta-endorphin was first obtained and then identified by immunoblotting and incubated with a quick-frozen section of young rat spinal cord. The use of a specific antibody with the immunoperoxidase reaction gave a morphological visualization of the beta-endorphin in the histological sections of the rat spinal cord.

Item Type:Article
ID Code:7804
Status:Published
Refereed:Yes
Uncontrolled Keywords:Avidin-biotin-peroxidase technique, immunological localization, beta-endorphin
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:EdiErmes
ISSN:0391-7258
Deposited On:23 Jul 2012 17:04

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