De Riu, Pier Luigi and Bitti, A. and Rubino, Salvatore and Virdis, G. and Fiori, Pier Luigi (1986) Localization of beta-endorphins in the rat spinal cord using avidin-biotin technology. Basic and Applied Histochemistry, Vol. 30 (4), p. 495-499. ISSN 0391-7258. Article.
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The avidin-biotin-peroxidase technique is proposed for the immunological localization of beta-endorphin in the rat spinal cord. A rabbit specific antibody anti-human beta-endorphin was first obtained and then identified by immunoblotting and incubated with a quick-frozen section of young rat spinal cord. The use of a specific antibody with the immunoperoxidase reaction gave a morphological visualization of the beta-endorphin in the histological sections of the rat spinal cord.
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