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The Cytoskeleton of hydatid cyst cultured cells and its sensitivity to inhibitors

Rubino, Salvatore and Fiori, Pier Luigi and Lubinu, Giuseppe and Monaco, Gianni and Cappuccinelli, Pietro Antonio (1983) The Cytoskeleton of hydatid cyst cultured cells and its sensitivity to inhibitors. European Journal of Cell Biology, Vol. 30 (2), p. 182-190. eISSN 1618-1298. Article.

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Cell cultures obtained from the germinal layer of hydatid cysts of the parasitic tapeworm Echinococcus granulosus were characterized with respect to their microtubule and microfilament systems. These were stained using monospecific antibodies against tubulin from sea urchin spermatozoa or sheep brain and against Dictyostelium discoideum actin as well as rhodamine conjugated phalloidin. The results show that the distribution of microtubules nad actin containing fibres of these cells is remarkably similar to that of mammalian cells both during interphase and mitosis. Hydatid cells, however, could not be stained with a specific antivimentin antibody. Indirect immunofluorescence with antitubulin antibodies of inhibitor treated cells shows that hydatid cell microtubules are sensitive to several antimicrotubular drugs including benzimidazole derivatives, colchicine, vinblastine, and griseofulvin.

Item Type:Article
ID Code:7803
Uncontrolled Keywords:Cytoskeleton, hydatid cysts, antimicrotubular drugs
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:Urban und Fischer
Deposited On:23 Jul 2012 16:21

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