Rocca, Stefano and Sanna, Maria Paola and Farigu, Serafina and Leoni, Antonio and Nieddu, Antonio Mario S. and Pirino, Salvatore and Appino, Simonetta (2005) Lesioni da MAP: indagine in situ su campioni di PTBC ovina pauci e multibacillare = MAP lesions: in situ investigation on samples of multibacillar and paucibacillar ovine PTBC. In: 59. Annual meeting of the Italian Society for Veterinary Sciences (SISVET), 21-24 September 2005, Viareggio, Italia. S.l., IVIS. p. 111-112. Conference or Workshop Item.
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To dimostrate efficaciously the role of MAP in the pathogenesis of sheepparatuberculosis and human-Crohn disease, five ovine-gut affected by multibacillar and two by paucibacillar PTBC were tested with different techniques of in-situ hybridization (ISH) and in-situ PCR (ISPCR). The best results, both in multibacillar than paucibacillar cases, were obtained by ISH with S89 and S92 probes. Quantity and quality of signals were remarkably increased by ISPCR, through P90 and P91 primers subsequently hybridizated with S89 and S92 probes. Our findings showed, by ISH or ISPCR, the presence of MAP in paucibacillar infections substained by Ziehl-Nilsen-negative agents.
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