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Genetic variation within Taenia multiceps in Sardinia, Western Mediterranean (Italy)

Varcasia, Antonio and Lightowlers, Marshall W. and Cattoli, Giovanni and Cancedda, Giovanna Maria and Canu, Sara and Garippa, Giovanni and Scala, Antonio (2006) Genetic variation within Taenia multiceps in Sardinia, Western Mediterranean (Italy). Parasitology Research, Vol. 99 (5), p. 622-626. eISSN 1432-1955. Article.

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DOI: 10.1007/s00436-006-0179-y

Abstract

Investigations were undertaken on Taenia multiceps to determine if genetic variation was present within the parasites of Sardinia (Italy). Forty samples were obtained from various locations of Sardinia and deoxyribonucleic acid (DNA) was extracted. Polymerase chain reaction (PCR) was performed on NADH dehydrogenase I (ND1) and cytochrome c subunit 1 (CO1) mitochondrial genes and amplicons were then sequenced and aligned with Bioedit software. Pairwise comparison between the ND1 sequences of the T. multiceps isolates showed differences ranging from 1.27 to 2.54% using an isolate obtained from Wales as an outgroup, while COI sequences showed within the samples coming from Sardinia a lesser degree of variability, ranging from 0.22 to 0.67%. Considering the two genes, it was possible to define at least three specific genetic variants in Sardinian samples, which we have termed Tm1, Tm2, and Tm3. This is the first description of genetic variability in T. multiceps. Further investigations will be required to understand to what extent the genetic variability described in this paper would be reflected also in phenotypic differences.

Item Type:Article
ID Code:7785
Status:Published
Refereed:Yes
Uncontrolled Keywords:Taenia multiceps, genetic variation, Sardinia
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/06 Parassitologia e malattie parassitarie degli animali
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Springer Berlin / Heidelberg
eISSN:1432-1955
Deposited On:16 Jul 2012 11:32

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