UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Reverse injection capillary electrophoresis UV detection for serotonin quantification in human whole blood

Zinellu, Angelo and Sotgia, Salvatore and Deiana, Luca and Carru, Ciriaco (2012) Reverse injection capillary electrophoresis UV detection for serotonin quantification in human whole blood. Journal of Chromatography B, Vol. 895–896 , p. 182-185. eISSN 1873-376X. Article.

Full text not available from this repository.

DOI: 10.1016/j.jchromb.2012.03.024

Abstract

We describe the first capillary electrophoresis UV detection method to measure serotonin in human whole blood (WB). Procedural parameters such as concentration and pH of run buffer and injection mode were investigated. The reverse injection allows to decrease the analysis time by injecting samples at the outlet end of the silica capillary close to the detection window, so reducing the migration distance. Thus, when a capillary with an effective length of 10 cm and a 400 mmol/L Tris phosphate as background electrolyte at pH 3.25 was used, the migration time of the serotonin peak was 2.6 min. These conditions gave a good reproducibility of migration times (CV, 0.77%) and peak areas (CV, 2.44%). Intra- and inter-assay CV were 3.85% and 7.32%, respectively, and the analytical recovery was between 96.8% and 99.4%.

Item Type:Article
ID Code:7765
Status:Published
Refereed:Yes
Uncontrolled Keywords:Serotonin, capillary electrophoresis, reverse injection, tris phosphate
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Azienda ASL1, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Elsevier
eISSN:1873-376X
Deposited On:12 Jul 2012 09:04

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page