Zedda, Marco and Lepore, Gianluca and Gadau, Sergio Domenico and Manca, Paolo and Farina, Vittorio (2004) Morphological and functional changes induced by the amino acid analogue 3-nitrotyrosine in mouse neuroblastoma and rat glioma cell lines. Neuroscience Letters, Vol. 363 (2), p. 190-193. ISSN 0304-3940. Article.
Full text not available from this repository.
The amino acid analogue 3-nitrotyrosine (3-NT) is formed in neural cells as a result of the intense stimulation of NMDA glutamate receptors. 3-NT is involved in the pathology of diverse neurodegenerative disorders. The aim of our work is to investigate the sensitivity of cultured neural and glial cells to 3-NT. We report the morphologicalchanges detected on mouse neuroblastoma (C1300) and rat glioma (C6) celllines cultured in a medium supplemented with different 3-NT concentrations. Western blot displayed a selective incorporation of 3-NT into a single protein that co-migrated with tubulin. Both celllines showed morphologicalchanges, nuclear suffering, decreased viability and growth inhibition (starting from 90 and 360 μM for C1300 and C6, respectively). Such effects were dose-dependent, though gliomacells showed severe alterations at higher 3-NT concentrations. Our results point out a higher 3-NT sensitivity in the neural cells studied in comparison with those of glial origin. The dramatic toxicity of 3-NT in neural cells suggests further investigations focused on the biochemical mechanisms at the roots of neurodegenerative diseases.
I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore
Repository Staff Only: item control page