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Morphological and functional changes induced by the amino acid analogue 3-nitrotyrosine in mouse neuroblastoma and rat glioma cell lines

Zedda, Marco and Lepore, Gianluca and Gadau, Sergio Domenico and Manca, Paolo and Farina, Vittorio (2004) Morphological and functional changes induced by the amino acid analogue 3-nitrotyrosine in mouse neuroblastoma and rat glioma cell lines. Neuroscience Letters, Vol. 363 (2), p. 190-193. ISSN 0304-3940. Article.

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DOI: 10.1016/j.neulet.2004.04.008

Abstract

The amino acid analogue 3-nitrotyrosine (3-NT) is formed in neural cells as a result of the intense stimulation of NMDA glutamate receptors. 3-NT is involved in the pathology of diverse neurodegenerative disorders. The aim of our work is to investigate the sensitivity of cultured neural and glial cells to 3-NT. We report the morphologicalchanges detected on mouse neuroblastoma (C1300) and rat glioma (C6) celllines cultured in a medium supplemented with different 3-NT concentrations. Western blot displayed a selective incorporation of 3-NT into a single protein that co-migrated with tubulin. Both celllines showed morphologicalchanges, nuclear suffering, decreased viability and growth inhibition (starting from 90 and 360 μM for C1300 and C6, respectively). Such effects were dose-dependent, though gliomacells showed severe alterations at higher 3-NT concentrations. Our results point out a higher 3-NT sensitivity in the neural cells studied in comparison with those of glial origin. The dramatic toxicity of 3-NT in neural cells suggests further investigations focused on the biochemical mechanisms at the roots of neurodegenerative diseases.

Item Type:Article
ID Code:7752
Status:Published
Refereed:Yes
Uncontrolled Keywords:3-Nitrotyrosine, neuroblastoma and glioma cell lines, morpho-functional changes, growth inhibition
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Elsevier
ISSN:0304-3940
Deposited On:09 Jul 2012 08:48

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