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D-glucose induces microtubular changes in C1300 neuroblastoma cell line through the incorporation of 3-nitro-L-tyrosine into tubulin

Gadau, Sergio Domenico and Lepore, Gianluca and Zedda, Marco and Manca, Paolo and Chisu, Valentina and Farina, Vittorio (2008) D-glucose induces microtubular changes in C1300 neuroblastoma cell line through the incorporation of 3-nitro-L-tyrosine into tubulin. Archives Italiennes de Biologie, Vol. 146 (2), p. 107-117. ISSN 0003-9829. Article.

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Abstract

The microtubular network of neurons is involved in several functions such as formation and tropism of cellular processes, cell division and intracellular transport. A lot of evidences testify that the microtubular network of neurons can be impaired by oxidative stress. A condition of oxidative stress is often possible when D-glucose overloads its metabolic pathway, resulting in an increase in reactive oxygen species and subsequent neurological disorders. The aim of this work was to check in undifferentiated mouse neuroblastoma cells (C1300) the possible oxidative effects of D-glucose on microtubules. Using a concentration of 110mM D-glucose, cell morphology, growth rate, viability and catalase activity were seriously altered. Noteworthy, an increase in 3-nitro-L-tyrosine and a downregulation of tubulins was found in D-glucose-exposed cells, whereas another cytoskeletal proteins, namely actin, did not show any changes. In conclusion, microtubular network can be impaired by D-glucose through specific nitrosative effects, suggesting a possible mechanism at the basis of hyperglycemia-induced neuronal damage.

Item Type:Article
ID Code:7743
Status:Published
Refereed:Yes
Uncontrolled Keywords:D-glucose, neuroblastoma cells (C1300), microtubular network
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Plus / Pisa University Press
ISSN:0003-9829
Deposited On:05 Jul 2012 10:20

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