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Identification of Fusarium oxysporum f. sp. basilici isolated from soil, basil seed, and plants by RAPD analysis

Chiocchetti, Annalisa and Ghignone, Stefano and Minuto, Andrea and Gullino, Maria Lodovica and Garibaldi, Angelo and Migheli, Quirico (1999) Identification of Fusarium oxysporum f. sp. basilici isolated from soil, basil seed, and plants by RAPD analysis. Plant Disease, Vol. 83 (6), p. 576-581. ISSN 0191-2917. Article.

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DOI: 10.1094/PDIS.1999.83.6.576

Abstract

Fifty-two isolates of Fusarium oxysporum, obtained from infected basil plants, seed, flower residues, and soil from different growing areas in Italy and Israel, were analyzed by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), coupled to a DNA extraction protocol from colonies grown on Fusarium-selective medium. In a pathogenicity assay, 35 isolates caused 32 to 92% disease on seedlings of the highly susceptible basil cultivar Fine verde, while 17 isolates were nonpathogenic on basil. Thirty of the F. oxysporum f. sp. basilici isolates obtained from soil or wilted plants gave identical amplification patterns using 31 different random primers. All tested primers allowed clear differentiation of F. oxysporum f. sp. basilici from representatives of other formae speciales and from nonpathogenic strains of F. oxysporum. RAPD profiles obtained from DNA of isolates extracted directly from cultures grown on Fusarium selective medium were identical to those obtained from DNA extracted from lyophilized mycelia

Item Type:Article
ID Code:77
Status:Published
Refereed:Yes
Uncontrolled Keywords:Diagnosis, DNA purification, Fusarium wilt, seed quality
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:The American Phytopathological Society
ISSN:0191-2917
Deposited On:18 Aug 2009 10:01

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