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Use of silicon and GaAs pixel detectors for digital autoradiography

Amendolia, Salvator Roberto and Beccherle, R. and Bertolucci, Ennio and Bisogni, Maria Giuseppina and Bottigli, Ubaldo and Campbell, Michael and Chesi, E. and Ciocci, Maria Agnese and Conti, Maurizio and Da Via, Cinzia and Del Guerra, Alberto and D'Auria, Saverio and Fantacci, Maria Evelina and Gambaccini, Mauro and Grossi, Gianfranco and Heijne, Erik and Mancini, E. and Marchesini, Roberta and Middelkamp, P. and O'Shea, V. and Randaccio, Paolo and Romeo, N. and Rosso, Valeria and Russo, Paola and Scharfetter, Luitwin and Smith, Kelvin and Snoeys, Walter and Stefanini, Arnaldo (1997) Use of silicon and GaAs pixel detectors for digital autoradiography. IEEE Transactions on Nuclear Science, Vol. 44 (3), p. 929-933. ISSN 0018-9499. Article.

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DOI: 10.1109/23.603779

Abstract

Solid state detectors made of Si (4.8×8 mm2) and GaAs (6.4×8 mm2) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with 32P. The system has shown a good minimum detectable activity per unit area of 2.10-4 cps mm-2, and has proved linear for a count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75×500 μm2 (Si-Omega2) and 50×500 μm2 (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).

Item Type:Article
ID Code:7642
Status:Published
Refereed:Yes
Uncontrolled Keywords:Si crystal detector, GaAs, Omega2/0mega3 electronics
Subjects:Area 02 - Scienze fisiche > FIS/07 Fisica applicata (a beni culturali, ambientali, biologia e medicina)
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:IEEE Nuclear and Plasma Sciences Society
ISSN:0018-9499
Deposited On:01 Jun 2012 09:30

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