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New antimalarial indolone-N-oxides, generating radical species, destabilize the host cell membrane at early stages of Plasmodium falciparum growth: role of band 3 tyrosine phosphorylation

Pantaleo, Antonella and Ferru, Emanuela and Vono, Rosa and Giribaldi, Giuliana and Lobina, Omar and Nepveu, Françoise and Ibrahim, Hany and Nallet, Jean-Pierre and Carta, Franco and Mannu, Franca Rita and Pippia, Proto Gavino and Campanella, Estela and Low, Philip S. and Turrini, Francesco Michelangelo (2012) New antimalarial indolone-N-oxides, generating radical species, destabilize the host cell membrane at early stages of Plasmodium falciparum growth: role of band 3 tyrosine phosphorylation. Free Radical Biology and Medicine, Vol. 52 (2), p. 527-536. eISSN 1873-4596. Article.

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DOI: 10.1016/j.freeradbiomed.2011.11.008

Abstract

Although indolone-N-oxide (INODs) genereting long-lived radicals possess antiplasmodial activity in the low-nanomolar range, little is known about their mechanism of action. To explore the molecular basis of INOD activity, we screened for changes in INOD-treated malaria-infected erythrocytes (Pf-RBCs) using a proteomics approach. At early parasite maturation stages, treatment with INODs at their IC50 concentrations induced a marked tyrosinephosphorylation of the erythrocyte membrane protein band 3, whereas no effect was observed in control RBCs. After INOD treatment of Pf-RBCs we also observed: (i) accelerated formation of membrane aggregates containing hyperphosphorylated band 3, Syk kinase, and denatured hemoglobin; (ii) dose-dependent release of microvesicles containing the membrane aggregates; (iii) reduction in band3phosphorylation, Pf-RBC vesiculation, and antimalarial effect of INODs upon addition of Syk kinase inhibitors; and (iv) correlation between the IC50 and the INOD concentrations required to induce band 3 phosphorylation and vesiculation. Together with previous data demonstrating that tyrosinephosphorylation of oxidized band 3 promotes its dissociation from the cytoskeleton, these results suggest that INODs cause a profound destabilization of the Pf-RBC membrane through a mechanism apparently triggered by the activation of a redox signaling pathway rather than direct oxidative damage.

Item Type:Article
ID Code:7624
Status:Published
Refereed:Yes
Uncontrolled Keywords:Red blood cells, tyrosine phosphorylation, Plasmodium falciparum, band 3, oxidative damage, free radicals
Subjects:Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Nurex Bioresearch Sassari, Sassari
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Elsevier
eISSN:1873-4596
Deposited On:25 May 2012 09:04

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