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In vivo and in vitro evidence for an association between the route-specific transmission of HHV-8 and the virus genotype

Matteoli, Barbara and Broccolo, Francesco and Scaccino, Antonio and Cottoni, Francesca Maria Giovanna and Angeloni, Antonio and Faggioni, Alberto and Ceccherini Nelli, Luca (2012) In vivoand in vitro evidence for an association between the route-specific transmission of HHV-8 and the virus genotype. Journal of Medical Virology, Vol. 84 (5), p. 786-791. eISSN 1096-9071. Article.

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DOI: 10.1002/jmv.23246

Abstract

The study was performed to determine if there is an association between the genotype and transmission of HHV-8 types A and C. These HHV-8 subtypes are prevalent in the area of North of Sardinia, which is an island off west Italy's mainland that has a high HHV-8 seroprevalence (35%). Blood and saliva samples from 30 patients with classic Kaposi's sarcoma who were lifetime residents of North Sardinia were analyzed to identify the HHV-8 genotype and quantitate the viral load. Genotype A, especially A1 subtype, was found more frequently (9/30 patients) and had a significantly higher viral load in saliva compared to blood (P = 0.029), where type C was found more frequently but with a viral load lower than 103 copies/ml. To determine if there is a correlation between the viral genotype and cellular tropism, type A1 and C3 HHV-8 viral particles were obtained from cell lines BCBL1 and BC3 infected chronically with HHV-8 A1 and C3 genotypes respectively and used to infect HEK293 epithelial origin cells and PBMCs in vitro. The data indicate that the A1 HHV-8 genotype is tropic and replicates at higher levels in the epithelial cell lines.

Item Type:Article
ID Code:7550
Status:Published
Refereed:Yes
Uncontrolled Keywords:HHV-8, classic KS, HHV-8 genotype, transmission, cellular tropism
Subjects:Area 06 - Scienze mediche > MED/35 Malattie cutanee e veneree
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
Publisher:Wiley-Liss
eISSN:1096-9071
Deposited On:10 May 2012 15:39

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