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Dual asymmetric-flow microdialysis for in vivo monitoring of brain neurochemicals

Bazzu, Gianfranco and Biosa, Alice and Farina, Donatella and Spissu, Ylenia and Dedola, Sonia and Calia, Giammario and Puggioni, Giulia Maria Grazia and Rocchitta, Gaia Giovanna Maria and Migheli, Rossana and Desole, Maria Speranza and Serra, Pier Andrea (2011) Dual asymmetric-flow microdialysis for in vivo monitoring of brain neurochemicals. Talanta, Vol. 85 (4), p. 1933-1940. eISSN 1873-3573. Article.

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DOI: 10.1016/j.talanta.2011.07.018


Microdialysis is an extensively used technique for both in vivo and in vitro experiments, applicable to animal and human studies. In neurosciences, the in vivo microdialysis is usually performed to follow changes in the extracellular levels of substances and to monitor neurotransmitters release in the brain of freely moving animals. Catecholamines, such as dopamine and their related compounds, are involved in the neurochemistry and in the physiology of mental diseases and neurological disorders. It is generally supposed that the brain's energy requirement is supplied by glucose oxidation. More recently, lactate was proposed to be the metabolic substrate used by neurons during synaptic activity. In our study, an innovative microdialysis approach for simultaneous monitoring of catecholamines, indolamines, glutamate and energy substrates in the striatum of freely moving rats, using an asymmetric perfusion flow rate on microdialysis probe, is described. As a result of this asymmetric perfusion, two samples are available from the same brain region, having the same analytes composition but different concentrations. The asymmetric flow perfusion could be a useful tool in neurosciences studies related to brain's energy requirement, such as toxin-induced models of Parkinson's disease.

Item Type:Article
ID Code:7503
Uncontrolled Keywords:In vivo microdialysis, liquid chromatography, dopamine, glutamate, brain bioenergetics, glucose, lactate, pyruvate, ascorbic acid, rat striatum
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Deposited On:08 May 2012 09:43

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