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A New multiplex PCR for the detection of hbl genes in strains of the "Bacillus cereus group"

Corona, A. and Fois, M. P. and Mazzette, Rina and De Santis, Enrico Pietro Luigi (2003) A New multiplex PCR for the detection of hbl genes in strains of the "Bacillus cereus group". Veterinary Research Communications, Vol. 27 (Suppl. 1), p. 679-682. eISSN 1573-7446. Article.

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DOI: 10.1023/B:VERC.0000014246.87789.75

Abstract

The Bacillus cereus group includes the species B. cereus, B. thuringensis, B. anthracis, B. mycoides, B. pseudomycoides and B. weihenstephanensis which are characterized by remarkable genetic affinities (Ash et al., 1991; Carlson et al., 1994; Henderson et al., 1995; Stephan, 1996; Borin et al., 1997; Nilsson et al., 1998; Shangkuan et al., 2000). These micro-organisms are responsible for foodborne disease through the production of several enterotoxins (Prüß et al., 1999) of which haemolysin BL (HBL) is the most studied. HBL is a three-component protein, including two lytic (L1 and L2) and one binding protein (B) and all three components must be simultaneously present to consider B. cereus group strains as potential pathogens (in’t Veld et al., 2001). HBL has haemolytic and dermonecrotic activity which is responsible for the increase in vascular permeability and it is considered the primary virulence factor in the diarrhoic syndrome (Beecher and Wong, 1994). The transcription of complex HBL is codified from the operone hbl. Traditional PCR, by means of specific primers (Hansen and Hendriksen, 2001), can only detect single genes (hblA, hblD, hblC) encoding the components of HBL (B, L1 e L2). The identification of the genes could be faster using only one PCR reaction. In the present study we developed a multiplex PCR for the detection of operon hbl genes, both on reference and wild strains isolated from ovine milk and ‘ricotta’ cheese.

Item Type:Article
ID Code:7378
Status:Published
Refereed:Yes
Uncontrolled Keywords:B. cereus group, enterotoxin HBL, multiplex PCR
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Springer
eISSN:1573-7446
Copyright Holders:© 2003 Kluwer Academic Publishers
Deposited On:13 Apr 2012 10:14

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