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Antibiotic resistance assessment in S. aureus strains isolated from raw sheep’s milk cheese

Spanu, Vincenzo and Virdis, S. and Scarano, Christian and Cossu, Francesca and De Santis, Enrico Pietro Luigi and Cosseddu, A. M. (2010) Antibiotic resistance assessment in S. aureus strains isolated from raw sheep’s milk cheese. Veterinary Research Communications, Vol. 34 (Suppl. 1), p. 87-90. eISSN 1573-7446. Article.

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DOI: 10.1007/s11259-010-9404-3


In vitro activities of 16 antibiotics were tested against 36 Staphylococcus aureus (SA) strains isolated from raw sheep’s milk cheese from six dairies. The minimum inhibitory concentration (MIC) was determined using a broth microdilution method (CLSI). All 36 isolates were analyzed for the presence of the accessory gene regulator gene, agr (I–IV), and genes encoding resistance to methicillin (mecA), erythromycin (ermA), penicillin (blaZ), and vancomycin (vanA-B). The isolates were also analyzed for similarities in pulsed-field gel electrophoresis (PFGE) patterns. SA strains showed resistance to ampicillin (36.1%), penicillin (33.3%), tetracycline (11.1%), and cloxacillin (2.8%) but were susceptible (≥94.4%) to 12 out of 16 tested antimicrobials. The overall susceptibility of the strains to oxacillin, vancomycin, and erythromycin was confirmed by the absence of the mecA, vanA-B, and ermA genes. The PFGE results showed that 32 strains belonged to 10 different clusters (P1–P10) while four strains were untypeable.

Item Type:Article
ID Code:7347
Uncontrolled Keywords:S. aureus, sheep cheese, antibiotic resistance, MIC, genes
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Additional Information:Paper presented at the "63. Annual meeting of the Italian Society for Veterinary Sciences (SISVET), Udine 2009".
Deposited On:10 Apr 2012 10:14

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