Scarano, Christian and Dalmasso, Alessandra and Spanu, Carlo and Spanu, Vincenzo and Cossu, Francesca and Mura, Emilio and Penna, Cecilia and Virdis, Salvatore and De Santis, Enrico Pietro Luigi (2011) Prevalenza e identificazione di Vibrio spp. in orate di allevamento = Prevalence and identification of Vibrio spp. isolated on aquacultured gilthead sea bream. Italian Journal of Food Safety = Rivista dell'Associazione Italiana dei Veterinari Igienisti, Vol. 1 , p. 115-119. ISSN 2239-7132. Article.
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The aim of the study was to investigate the prevalence of Vibrio spp isolated from gilthead sea bream (Sparus aurata) farmed on sea cages and to identify and characterize the pathogen by molecular techniques. Eighty fish were collected from two hatcheries located on the North-Est Sardinian Mediterranean coast, and microbiological analysis were performed on different body parts such as skin, gills, muscle and intestinal tract. Subsequently 100 pure colonies with typical morphology and phenotypic characteristics were selected and submitted to the molecular identification. The analysis on the prevalence of Vibrio spp showed the effect of the hatchery rearing system (P<0.001), of the date of sampling (P<0.001), and of the body part (P<0.001). All the strains selected were confirmed to be members of the genus Vibrio spp by the molecular method/techinique/identification, whereas the rpoA gene sequence analyses allowed to identify 89 strains belonging to the species Vibrio harveyi, 6 to V. diabolicus, 2 to V. parahaemolyticus and 1 to V. mediterranei.
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