Vigo, Daniele and Torre, Marina L. and Faustini, Massimo and Russo, Vincenzo and Norberti, Roberta and Villani, Simona and Asti, Annalia and Bini, Pier Paolo and Conte, Ubaldo (2004) Barium alginate capsules for 3D immobilisation of living cells: morphology, membrane properties and permeability. Journal of Drug Delivery Science and Technology, Vol. 14 (3), p. 167-172. ISSN 1157-1489. Article.
Encapsulation in a barium alginate membrane is a promising strategy to obtain a three dimensional culture of living cells: membrane properties are crucial for a realistic clinical application. A one-step encapsulation technique, recently developed for controlled release of boar semen, was employed to prepare barium alginate and protamine-alginate membranes: permeability to two model molecules (haemoglobin and glucose) was evaluated. Capsules were evaluated for technological properties and scanning electron microscopy was used to examine the external morphology of the capsules and the 3D distribution of the cells within the core. The results indicate that 3D arrangement and cell shape are maintained, capsule dimensions and mechanical properties can be modulated, as well as their permeability to model molecules such as haemoglobin and glucose.
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