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Contractile regulatory proteins tropomyosin and troponin-T as indicators of the modulatory role of retinoic acid

Vélez, C. and Aránega, Amelia Eva and Marchal Corrales, Juan Antonio and Prados Salazar, José Carlos and Melguizo, Consolacion and Carrillo Delgado, Esmeralda Esperanza and Boulaiz, Houria and Madeddu, Roberto Beniamino and Sánchez Montesinos, I. (2003) Contractile regulatory proteins tropomyosin and troponin-T as indicators of the modulatory role of retinoic acid. Cells Tissues Organs, Vol. 175 (1), p. 25-33. eISSN 1422-6421. Article.

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DOI: 10.1159/000073434

Abstract

Retinoic acid (RA), the active metabolite of vitamin A, plays a significant role in regulating cardiac form and function throughout the life of the organism. Both cardiac morphogenesis and myocardial differentiation are affected by alterations in RA homeostasis. In order to test the effect of all-trans RA and 13-cis RA on cardiomyocyte differentiation, we studied the level and the subcellular compartmentalization of α-tropomyosin and troponin-T proteins in cultures of chick embryo cardiomyocytes obtained from Hamburger and Hamilton’s (HH) stage 22, 32 and 40 embryos. The retinoids increased the levels of α-tropomyosin and troponin-T in the cytoplasmic and cytoskeletal fractions of cells at all three stages of development. The greatest increases in α-tropomyosin occurred in the cytoplasmic fraction in HH22 cells cultured for 24 h with all-trans RA or 13-cis RA, whereas the greatest increases in troponin-T were found in the cytoplasmic fraction of HH32 cells exposed to retinoids for 24 h. In cultures treated for 48 h with retinoids, the levels of α-tropomyosin and troponin-T showed significant increases in the cytoplasmic compartment of cells treated in HH32-with respect to the control values. These findings are further evidence that RA plays a modulating role in the formation and reorganization of sarcomeric proteins during the process of cardiomyocyte maturation.

Item Type:Article
ID Code:7285
Status:Published
Refereed:Yes
Uncontrolled Keywords:Retinoic acid, tropomyosin, troponin-T, cardiomyocytes, differentiation, cell culture, chick
Subjects:Area 05 - Scienze biologiche > BIO/17 Istologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:S. Karger
eISSN:1422-6421
Deposited On:23 Mar 2012 10:18

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