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Tagging the signatures of domestication in common bean (Phaseolus vulgaris) by means of pooled DNA samples

Papa, Roberto and Bellucci, Elisa and Rossi, Monica and Leonardi, Stefano and Rau, Domenico and Gepts, Paul and Nanni, Laura and Attene, Giovanna (2007) Tagging the signatures of domestication in common bean (Phaseolus vulgaris) by means of pooled DNA samples. Annals of Botany, Vol. 100 (5), p. 1039-1051. eISSN 1095-8290. Article.

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DOI: 10.1093/aob/mcm151

Abstract

Background and Aims: The main aim of this study was to use an amplified fragment length polymorphism (AFLP)-based, large-scale screening of the whole genome of Phaseolus vulgaris to determine the effects of selection on the structure of the genetic diversity in wild and domesticated populations. Methods: Using pooled DNA samples, seven each of wild and domesticated populations of P. vulgaris were studied using 2506 AFLP markers (on average, one every 250 kb). About 10 % of the markers were also analysed on individual genotypes and were used to infer allelic frequencies empirically from bulk data. In both data sets, tests were made to determine the departure from neutral expectation for each marker using an FST-based method. Key Results: The most important outcome is that a large fraction of the genome of the common bean (16 %; P <0·01) appears to have been subjected to effects of selection during domestication. Markers obtained in individual genotypes were also mapped and classified according to their proximities to known genes and quantitative trait loci (QTLs) of the domestication syndrome. Most of the markers that were found to be potentially under the effects of selection were located in the proximity of previously mapped genes and QTLs related to the domestication syndrome. Conclusions: Overall, the results indicate that in P. vulgaris a large portion of the genome appears to have been subjected to the effects of selection, probably because of linkage to the loci selected during domestication. As most of the markers that are under the effects of selection are linked to known loci related to the domestication syndrome, it is concluded that population genomics approaches are very efficient in detecting QTLs. A method based on bulk DNA samples is presented that is effective in pre-screening for a large number of markers to determine selection signatures.

Item Type:Article
ID Code:702
Status:Published
Refereed:Yes
Uncontrolled Keywords:AFLP, domestication, Phaseolus vulgaris, population genomics, QTLs, selection.
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
Publisher:Oxford University Press on behalf of the Annals of Botany Company
eISSN:1095-8290
Copyright Holders:© 2007 The Author(s)
Deposited On:18 Aug 2009 10:03

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