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Cadmium influences the 5-Fluorouracil cytotoxic effects on breast cancer cells

Asara, Yolande and Marchal Corrales, Juan Antonio and Bandiera, Pasquale and Mazzarello, Vittorio Lorenzo Giuseppe and Delogu, Lucia Gemma and Sotgiu, Maria Alessandra and Montella, Andrea Costantino Mario and Madeddu, Roberto Beniamino (2012) Cadmium influences the 5-Fluorouracil cytotoxic effects on breast cancer cells. European Journal of Histochemistry, Vol. 56 (1), e1. eISSN 2038-8306. Article.

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DOI: 10.4081/ejh.2012.e1

Abstract

The aim of the research was to evaluate a heavy metal, Cadmium (Cd), which was used to produce alterations in human breast cancer cell line MCF-7. Moreover, we analyzed both immunohistochemical and ultrastructural alterations induced by the antineoplastic drug, 5-Fluorouracil (5-FU), after exposure to different concentrations of Cadmium. Also, we compared the effects of these compounds on actin and tubulin cytoskeleton proteins. Under ultramicroscopic observation, control cells looked polymorphous with filopodia. In cells already treated with small concentrations of Cd, after brief times of incubation, we observed an intense metabolic activity with larger, clearer, and elongated mitochondria characterized by thin and numerous dilated cristae. 5-FU-treated cells showed cytotoxicity signs with presence of pore-like alterations in the cell membrane and evident degeneration of cytoplasm and cell nuclei. The addition of 5-FU (1.5 μM) to the cells treated with Cd (5 μM-20 μM) did not induce significant ultrastructural changes in comparison with cells treated only with Cd. In Cd+5FU-treated cells mitochondria with globular aspect and regular cristae indicated the active metabolic state. In cells treated only with Cd we observed alterations in actin distribution, while tubulin branched out throughout the cytoplasm. With the association of Cd+5FU, we observed less morphological alterations in both tubulin and actin cytoskeleton proteins. Although the mechanism remains unknown at present, our findings suggest that Cd prevents the cytotoxic effect of 5-FU on breast cancer cells. These preliminary results could have an important clinical application in patients with breast cancer.

Item Type:Article
ID Code:7012
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cadmium, 5-Fluorouracil, MCF-7 cells
Subjects:Area 05 - Scienze biologiche > BIO/17 Istologia
Area 05 - Scienze biologiche > BIO/16 Anatomia umana
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:PAGEPress
eISSN:2038-8306
Copyright Holders:©Copyright Y. Asara et al., 2012 Licensee PAGEPress, Italy
Deposited On:24 Jan 2012 10:51

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