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mtDNA control region and D-HPLC analysis: a method to evaluate the mating system in Syngnathidae (Teleostei)

Sanna, Daria and Addis, Alberto and Biagi, Francesca and Motzo, Costantino and Carcupino, Marcella and Francalacci, Paolo (2008) mtDNA control region and D-HPLC analysis: a method to evaluate the mating system in Syngnathidae (Teleostei). Marine Biology, Vol. 153 (3), p. 269-275. eISSN 1432-1793. Article.

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DOI: 10.1007/s00227-007-0810-1

Abstract

Mitochondrial DNA (mtDNA) has been used extensively in animals for phylogenetic and population studies but it has been applied only occasionally to mating systems analysis, where nuclear markers are usually preferred. We used denaturing-high performance liquid chromatography (D-HPLC) to detect mitochondrial DNA polymorphisms to assess the genetic mating system in Syngnathus abaster. Our study of ten pregnant males, revealed polygyny in 30% of the males, who carried embryos originating from multiple females. In addition, 30% of the pregnant males carried embryos with the same mitochondrial haplotype. This is not sufficient to demonstrate polyandry but allows a rapid selection and a reduced sample size for further studies. In conclusion, the proposed technique is time- and cost-effective, allows the certain identification of polygyny and provides useful information to study polyandry.

Item Type:Article
ID Code:697
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mitochondrial DNA (mtDNA), denaturing-high performance liquid chromatography (D-HPLC), Syngnathus abaster, mating system
Subjects:Area 05 - Scienze biologiche > BIO/05 Zoologia
Area 05 - Scienze biologiche > BIO/18 Genetica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Zoologia e genetica evoluzionistica
Publisher:Springer Berlin / Heidelberg
eISSN:1432-1793
Deposited On:18 Aug 2009 10:03

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