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Factors affecting S-homocysteinylation of LDL apoprotein B

Zinellu, Angelo and Zinellu, Elisabetta and Sotgia, Salvatore and Formato, Marilena and Cherchi, Gian Mario and Deiana, Luca and Carru, Ciriaco (2006) Factors affecting S-homocysteinylation of LDL apoprotein B. Clinical Chemistry, Vol. 52 , p. 2054-2059. eISSN 1530-8561. Article.

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DOI: 10.1373/clinchem.2006.071142

Abstract

Background: Hyperhomocysteinemia is an important risk factor for vascular disease and atherosclerosis, but the mechanisms by which homocysteine exerts its deleterious effects are not known. Because oxidation and/or homocysteinylation may increase atherogenicity of LDL, we investigated S-homocysteinylation of LDL as a possible contributor to atherosclerosis pathogenesis. Methods: We used capillary electrophoresis to measure LDL-bound thiols [homocysteine, cysteine (Cys), cysteinylglycine, glutathione, and glutamylcysteine] in 104 healthy study participants We also assessed total plasma thiol concentrations and lipid profiles. Results: Our data suggest that apoprotein B (apoB)-cysteinylglycine (CysGly), apoB-Hcy, and apoB-Cys concentrations are markedly higher in men than in women. The percentage of CysGly and glutathione on apoB was higher than that of the same thiols in plasma, whereas the other thiols were markedly less prevalent in lipoprotein than in plasma. Pearson correlation showed that among all thiols, only total plasma Hcy is related to apoB-Hcy concentrations. Multiple correlation analysis confirmed that total Hcy was the most important determinant of apoB-Hcy. Age and LDL cholesterol also showed positive associations, but Cys and, mainly, CysGly were negatively associated with apoB-Hcy concentrations. Conclusions: apoB-Hcy derivative formation is mainly dependent on total homocysteine concentration. Increased cholesterol concentrations are related to increased apoB-Hcy. CysGly seems to compete with Hcy for binding to LDL apoprotein, suggesting that CysGly may protect against atherosclerosis by decreasing the concentrations of Hcy transferred by LDL from plasma to endothelial and subendothelial spaces.

Item Type:Article
ID Code:692
Status:Published
Refereed:Yes
Uncontrolled Keywords:Hyperhomocysteinemia, atherosclerosis, homocysteine, apoprotein B (apoB), low-density lipoprotein (LDL)
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Association for Clinical Chemistry
eISSN:1530-8561
Deposited On:18 Aug 2009 10:03

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