Zara, Giacomo and Budroni, Marilena and Mannazzu, Ilaria Maria and Zara, Severino (2011) Air–liquid biofilm formation is dependent on ammonium depletion in a Saccharomyces cerevisiae flor strain. Yeast, Vol. 28 (12), p. 809-814. eISSN 1097-0061. Article.
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Air–liquid biofilm formation appears to be an adaptive mechanism that promotes foraging of Saccharomyces cerevisiae flor strains in response to nutrient starvation. The FLO11 gene plays a central role in this phenotype as its expression allows yeast cells to rise to the liquid surface. Here, we investigated the role of ammonium depletion in air–liquid biofilm formation and FLO11 expression in a S. cerevisiae flor strain. The data obtained show that increasing ammonium concentrations from 0 to 450 m m reduce air–liquid biofilm in terms of biomass and velum formation and correlate with a reduction of FLO11 expression. Rapamycin inhibition of the TOR pathway and deletion of RAS2 gene significantly reduced biofilm formation and FLO11 expression. Taken together, these data suggest that ammonium depletion is a key factor in the induction of air–liquid biofilm formation and FLO11 expression in S. cerevisiae flor strains.
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