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Recovery of mutants impaired in pathogenicity after transposition of Impala in Fusarium oxysporum f. sp. melonis

Migheli, Quirico and Steinberg, Christian and Davière, Jean-Michel and Olivain, Chantal and Gerlinger, Catherine and Gautheron, Nadine and Alabouvette, Claude and Daboussi, Marie-Josée (2000) Recovery of mutants impaired in pathogenicity after transposition of Impala in Fusarium oxysporum f. sp. melonis. Phytopathology, Vol. 90 (11), p. 1279-1284. ISSN 0031-949X. Article.

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DOI: 10.1094/PHYTO.2000.90.11.1279

Abstract

The ability of transposon impala to inactivate genes involved in pathogenicity was tested in Fusarium oxysporum f. sp. melonis. Somatic excision of an impala copy inserted in the nitrate reductase-encoding niaD gene was positively selected through a phenotypic assay based on the restoration of nitrate reductase activity. Independent excision events were analyzed molecularly and shown to carry reinsertedimpala in more than 70% of the cases. Mapping of reinserted impala elements on large NotI-restriction fragments showed that impala transposes randomly. By screening 746 revertants on plants, a high proportion (3.5%) of mutants impaired in their pathogenic potential was recovered. According to the kinetics of wilt symptom development, the strains that were impaired in pathogenicity were clustered in three classes: class 1 grouped two strains that never induced Fusarium wilt symptoms on the host plant; class 2 and class 3 grouped 15 and 9 revertants which caused symptoms more than 50 and 30 days after inoculation, respectively. The first results demonstrate the efficiency of transposition in generating mutants affected in pathogenicity, which are usually difficult to obtain by classical mutagenesis, and open the possibility to clone the altered genes with impala as a tag.

Item Type:Article
ID Code:69
Status:Published
Refereed:Yes
Uncontrolled Keywords:Melon, transposon tagging
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:The American Phytopathological Society
ISSN:0031-949X
Deposited On:18 Aug 2009 10:01

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