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Caratterizzazione fenotipica e molecolare di batteri azotofissatori simbionti associati a leguminose arbustive della Sardegna

Loi, Antonella (2012) Caratterizzazione fenotipica e molecolare di batteri azotofissatori simbionti associati a leguminose arbustive della Sardegna. Doctoral Thesis.

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Abstract

This work analyses the diversity of rhizobia associated to nodules of different leguminous shrubs in Sardinia, to date almost unknown. Seventy-eight rhizobial strains were isolated from root nodules of Astragalus terracianoi, Cytisus villosus, Coronilla valentina and Genista sardoa.
This collection was characterized by genomic and phenotypic features. Phylogenies were inferred from their 16S and three symbiotic genes (nodA, nodC and nifH). The genetic analysis revealed that the rhizobia isolated from Cytisus villosus. nodules belong to genera Bradyrhizobium, and that the rhizobia isolated from Astragalus terracianoi and Coronilla valentina nodules belong to genera Mesorhizobium, and that the rhizobia isolated from Genista sardoa nodules belong to genera Rhizobium, Phyllobacterium and Bradyrhizobium. The Microplate GenIII BiologTM has allowed a metabolic fingerprinting of isolates by using 71 carbon sources and 23 Chemical Sensitivity Assays.
The symbiotic tests showed differences among all rhizobia isolates with respect to their effectiveness and infectivity; for example, as with Genista sardoa, that had been infected from a single strain or as with Cytisus villosus, that although had been nodulated by all inoculated strains, did not produce dry weights different from the control plants. Moreover, it has been identified to be the best interaction microsymbiont-host plant that could be utilized in a revegetation project.like (as) potential strategy in revegetation project.

Item Type:Doctoral Thesis
ID Code:6783
Contributors:Deiana, Pietrino
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Rhizobia, shrubs, diversity, phylogeny, Sardinia
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Cicli, scuole e corsi:Ciclo 23 > Scuole > Scienze dei sistemi agrari e forestali e delle produzioni alimentari > Monitoraggio e controllo degli ecosistemi forestali in ambiente mediterraneo
Deposited On:28 Mar 2012 09:11

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