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Intraepithelial and interstitial deposition of pathological prion protein in kidneys of scrapie-affected sheep

Ligios, Ciriaco and Cancedda, Giovanna Maria and Margalith, Ilan and Santucciu, Cinzia and Madau, Laura and Maestrale, Caterina and Basagni, Massimo and Saba, Mariangela and Heikenwalder, Mathias (2007) Intraepithelial and interstitial deposition of pathological prion protein in kidneys of scrapie-affected sheep. PLoS One, Vol. 2 (9), p. e859. ISSN 1932-6203. Article.

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DOI: 10.1371/journal.pone.0000859

Abstract

Prions have been documented in extra-neuronal and extra-lymphatic tissues of humans and various ruminants affected by Transmissible Spongiform Encephalopathy (TSE). The presence of prion infectivity detected in cervid and ovine blood tempted us to reason that kidney, the organ filtrating blood derived proteins, may accumulate disease associated PrPSc. We collected and screened kidneys of experimentally, naturally scrapie-affected and control sheep for renal deposition of PrPSc from distinct, geographically separated flocks. By performing Western blot, PET blot analysis and immunohistochemistry we found intraepithelial (cortex, medulla and papilla) and occasional interstitial (papilla) deposition of PrPSc in kidneys of scrapie-affected sheep. Interestingly, glomerula lacked detectable signals indicative of PrPSc. PrPSc was also detected in kidneys of subclinical sheep, but to significantly lower degree. Depending on the stage of the disease the incidence of PrPSc in kidney varied from approximately 27% (subclinical) to 73.6% (clinical) in naturally scrapie-affected sheep. Kidneys from flocks without scrapie outbreak were devoid of PrPSc. Here we demonstrate unexpectedly frequent deposition of high levels of PrPSc in ovine kidneys of various flocks. Renal deposition of PrPSc is likely to be a pre-requisite enabling prionuria, a possible co-factor of horizontal prion-transmission in sheep.

Item Type:Article
ID Code:678
Status:Published
Refereed:Yes
Uncontrolled Keywords:Prions, Transmissible Spongiform Encephalopathy (TSE), PRion Protein SCrapie (PrPSc)
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
Publisher:Public Library of Science
ISSN:1932-6203
Additional Information:Pubblicato online il 12 settembre 2007.
Deposited On:18 Aug 2009 10:02

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