titoli, abstracts, parole chiave >>>
Mycobacterium avium subsp. paratuberculosis protein MAP 3865c may mimic the pancreatic islet autoantigen Znt8 relevant in type 1 diabetes

Masala, Speranza (2012) Mycobacterium aviumsubsp. paratuberculosis protein MAP 3865c may mimic the pancreatic islet autoantigen Znt8 relevant in type 1 diabetes. Doctoral Thesis.

Full text disponibile come PDF Richiede visualizzatore di PDF come GSview, Xpdf o Adobe Acrobat Reader


The environmental factors at play in the pathogenesis of type 1 diabetes (T1D) remain enigmatic. Mycobacterium avium subspecies paratuberculosis (MAP) is transmitted from dairy herds to humans through food contamination. MAP causes an asymptomatic infection which is highly prevalent in Sardinian T1D patients compared with type 2 diabetes (T2D) and healthy controls. Moreover, MAP elicits humoral responses against several mycobacterial proteins. We searched whether antibodies (Abs) against one of these proteins, namely MAP3865c, which displays a sequence homology with the β-cell protein zinc transporter 8 (ZnT8) could be cross-reactive with ZnT8 epitopes. To this end, Ab responses against MAP3865c were analyzed in Sardinian T1D, T2D and healthy subjects using indirect ELISA. Abs against MAP3865c recognized two immunodominant transmembrane epitopes in 52-65% of T1D patients, but only in 5-7% of T2D and 3-5% of healthy controls. There was a linear correlation between titers of anti-MAP3865c and anti-ZnT8 Abs targeting these two homologous epitopes, and pre-incubation of sera with ZnT8 epitope peptides blocked binding to the corresponding MAP3865c peptides. These results demonstrate that Abs recognizing MAP3865c epitopes cross-react with ZnT8, possibly underlying a molecular mimicry mechanism which may precipitate T1D in MAP-infected individuals.

Item Type:Doctoral Thesis
ID Code:6656
Contributors:Sechi, Leonardo Antonio
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Autoimmunity, environment, MAP, mimicry, Slc30A8, Sardinia
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Cicli, scuole e corsi:Ciclo 24 > Scuole > Scienze biomolecolari e biotecnologiche > Microbiologia molecolare e clinica
Deposited On:27 Mar 2012 15:13

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page