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E phage gene transfection associated to chemotherapeutic agents increases apoptosis in lung and colon cancer cells

Rama, Ana R. and Prados Salazar, José Carlos and Melguizo, Consolacion and Álvarez, Pablo J. and Ortiz, Raúl and Madeddu, Roberto Beniamino and Aranega Jiménez, Antonia (2011) E phage gene transfection associated to chemotherapeutic agents increases apoptosis in lung and colon cancer cells. Bioengineered Bugs, Vol. 2 (3), p. 163-167. eISSN 1949-1026. Article.

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DOI: 10.4161/bbug.2.3.15533

Abstract

The limited ability of conventional therapies to achieve the long-term survival of metastatic lung and colon cancer patients suggests the need for new treatment options. In this respect, genes encoding cytotoxic proteins have been proposed as a new strategy to enhance the activity of drugs, and combined therapies involving such genes and classical antitumoral drugs have been studied intensively. The E gene from phiX174 encodes a membrane protein with a toxic domain that leads to a decrease in tumour cell growth rates. Therefore, in order to improve the anti-tumour effects of currently used chemotherapeutic drugs on cancer cells, we investigated the association of the E suicide gene with these antineoplastic drugs. The E gene has antitumoral effects in both lung and colon cancer cells. In addition, expression of this gene induces ultrastructural changes in lung cancer transfected cells (A-549), although the significance of these changes remains unknown. The effect of combined therapy (gene and cytotoxic therapy) enhances the inhibition of tumour cell proliferation in comparison to single treatments. Indeed, our in vitro results indicate that an experimental therapeutic strategy based on this combination of E gene therapy and cytotoxic drugs may result in a new treatment strategy for patients with advanced lung and colon cancer.

Item Type:Article
ID Code:6599
Status:Published
Refereed:Yes
Uncontrolled Keywords:Gene therapy, gene E, cytotoxic therapy, colon cancer, lung cancer
Subjects:Area 05 - Scienze biologiche > BIO/17 Istologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Landes Bioscience
eISSN:1949-1026
Deposited On:21 Nov 2011 09:47

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