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Transformants of Trichoderma longibrachiatum overexpressing the beta-1,4-endoglucanase gene egl1 show enhanced biocontrol of Pythium ultimum on cucumber

Migheli, Quirico and Gonzalez-Candelas, Luis and Dealessi, Laura and Camponogara, Andrea and Ramón-Vidal, Daniel (1998) Transformants of Trichoderma longibrachiatum overexpressing the beta-1,4-endoglucanase gene egl1 show enhanced biocontrol of Pythium ultimum on cucumber. Phytopathology, Vol. 88 (7), p. 673-677. ISSN 0031-949X. Article.

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DOI: 10.1094/PHYTO.1998.88.7.673

Abstract

Nine transformants of Trichoderma longibrachiatum with extra copies of the egl1 gene were studied for mitotic stability, endoglucanase production, and biocontrol activity against Pythium ultimum on cucumber seedlings. The transformants showed a significantly higher level of expression of the egl1 gene in comparison to the wild type under both inducing and noninducing growth conditions. Transformants with the egl1 gene under the control of a constitutive promoter had the highest enzymatic activity. Both the endoglucanase activity and the transforming sequences were stable under nonselective conditions. When applied to cucumber seeds sown in P. ultimum-infested soil, T. longibrachiatum transformants with increased inducible or constitutive egl1 expression generally were more suppressive than the wild-type strain.

Item Type:Article
ID Code:65
Status:Published
Refereed:Yes
Uncontrolled Keywords:Damping-off, biological control, lytic enzymes, rhizosphere.
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:The American Phytopathological Society
ISSN:0031-949X
Deposited On:18 Aug 2009 10:01

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