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Proteomic profiling of human melanoma metastatic cell line secretomes

Rocco, Micaela and Malorni, Livia and Cozzolino, Rosaria and Palmieri, Giuseppe and Rozzo, Carla and Manca, Antonella and Parente, Augusto and Chambery, Angela (2011) Proteomic profiling of human melanoma metastatic cell line secretomes. Journal of Proteome Research, Vol. 10 (10), p. 4703-4714. ISSN 1535-3893. eISSN 1535-3907. Article.

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DOI: 10.1021/pr200511f

Abstract

During the last few years, the incidence and mortality of human melanoma have rapidly increased. Metastatic spread of malignant melanoma is often associated with cancer progression with poor prognosis and survival. These processes are controlled by dynamic interactions between tumor melanocytes and neighboring stromal cells, whose deregulation leads to the acquisition of cell proliferation capabilities and invasiveness. It is increasingly clear that a key role in carcinogenesis is played by secreted molecules either by tumor and surrounding stromal cells. To address the issue of the proteins secreted during cancer progression, the proteomic profiling of secretomes of cancer cell lines from different melanoma metastases of the same patient (PE-MEL-41, PE-MEL-47, and PE-MEL-43) was performed by applying a shotgun LCMS/MS-based approach. The results provide a list of candidate proteins associated with the metastatic potential of PE-MEL melanoma cell lines. Among them, several matricellular proteins previously reported as involved in melanoma aggressiveness were identified (i.e., SPARC, osteopontin). In addition, the extracellular matrix protein 1 that stimulates proliferation and angiogenesis of endothelial cells as well as the fibronectin, involved in cell adhesion and motility, were identified. The present work provides the basis to clarify the complex extracellular protein networks implicated in human melanoma cell invasion, migration, and motility.

Item Type:Article
ID Code:6409
Status:Published
Refereed:Yes
Uncontrolled Keywords:Melanoma, metastasis, LC-MS, proteomic profiling, secretome
Subjects:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Area 05 - Scienze biologiche > BIO/18 Genetica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di chimica biomolecolare, Sassari
Publisher:American Chemical Society
ISSN:1535-3893
eISSN:1535-3907
Copyright Holders:© 2011 American Chemical Society
Deposited On:25 Aug 2011 14:27

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