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Prune melanoidins protect against oxidative stress and endothelial cell death

Posadino, Anna Maria and Cossu, Annalisa and Piga, Antonio <1965- > and Madrau, Monica Assunta and Del Caro, Alessandra and Colombino, Maria and Paglietti, Bianca and Rubino, Salvatore and Iaccarino, Ciro and Crosio, Claudia and Sanna, Bastiano and Pintus, Gianfranco (2011) Prune melanoidins protect against oxidative stress and endothelial cell death. Frontiers in Bioscience, Vol. E3 , p. 1034-1041. ISSN 1093-9946. Article.

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DOI: 10.2741/309

Abstract

The health-promoting effects of fruit and vegetable consumption are thought to be due to phytochemicals contained in fresh plant material. Whether processed plant foods provide the same benefits as unprocessed ones is an open question. Melanoidins from heat-processed plums (prunes) were isolated and their presence confirmed by hydroxymethylfurfural content and browning index. Oxidative-induced endothelial cell (EC) damage is the trigger for the development of cardiovascular diseases (CVD); therefore the potential protective effect of prune melanoidins on hydrogen peroxide-induced oxidative cell damage was investigated on human endothelial ECV304 cells. Cytoplasmic and mitochondrial redox status was assessed by using the novel, redox-sensitive, ratiometric fluorescent protein sensor (roGFP), while mitochondrial membrane potential (MMP) was investigated with the fluorescent dye, JC-1. Treatment of ECV304 cells with hydrogen peroxide dose-dependently induced both mitochondrial and cytoplasmic oxidation, in addition to MMP dissipation, with ensuing cell death. Pretreatment of ECV304 with prune melanoidins, significantly counteracted and ultimately abolished hydrogen peroxide elicited phenomena, clearly indicating that these polymers protect human EC against oxidative stress.

Item Type:Article
ID Code:6256
Status:Published
Refereed:Yes
Uncontrolled Keywords:Melanoidins, antioxidant activity, prunes
Subjects:Area 05 - Scienze biologiche > BIO/11 Biologia molecolare
Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 07 - Scienze agrarie e veterinarie > AGR/15 Scienze e tecnologie alimentari
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Frontiers in Bioscience
ISSN:1093-9946
Deposited On:13 Jul 2011 13:48

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