Calderini, Ornella and Carelli, Maria and Panara, Francesco and Biazzi, Elisa and Scotti, Carla and Tava, Aldo and Porceddu, Andrea and Arcioni, Sergio (2011) Collection of mutants for functional genomics in the legume Medicago truncatula. Plant Genetic Resources: characterization and utilization, Vol. 9 (2), p. 174-176. ISSN 1479-2621. eISSN 1479-263X. Article.
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We have established mutant collections of the model species Medicago truncatula according to current protocols. In particular, we used a transposon (Tnt1) tagging method and an ethyl methanesulfonate (EMS) mutagenesis approach (TILLING). The collections were subjected to both forward and reverse genetics screenings, and several mutants were isolated that affect plant traits (e.g. shoot, root developments, flower morphology, etc.) and also biosynthetic pathways of secondary compounds (saponins and tannins). Genes responsible for some of the mutations were cloned and further characterized.
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