Varcasia, Antonio and Tanda, B. and Giobbe, M. and Solinas, C. and Pipia, Anna Paola and Malgor, Ramiro and Carmona, Carlos and Garippa, Giovanni and Scala, Antonio (2011) Cystic Echinococcosis in Sardinia: farmers’ knowledge and dog infection in sheep farms. Veterinary Parasitology, Vol. 181 (2-4), p. 335-340. ISSN 0304-4017. Article.
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Cystic Echinococcosis (CE) is one of the most widespread parasitic diseases in Sardinia, the second largest Mediterranean island where almost 3,558,000 milk sheep were raised extensively. The aim of this survey was to evaluate the level of farmers’ knowledge on CE transmission, focusing on the role of human to facilitate the persistence of this zoonosis in Sardinia after 14 years after the last campaign against CE. The other goal of the survey is to update on presence of Echinococcus granulosus in its definitive hosts through three ELISA coproantigen tests. An interview was carried out with 172 farmers. The questionnaire was designed to include possible factors associated with the transmission of Echinococcosis: ownership and number of dogs, the use of anthelmintic drugs against dog cestode, frequency of anthelmintic treatment in dogs, home slaughtering and offal disposal. Individual faecal samples were retrieved from 300 dogs, and after a preliminary macroscopic examination to discover adult worms and/or proglottids, was submitted to copromicroscopic examination. Coproantigens were then extracted according to the protocol described by Allan et al. (1992), and subsequently stored at −20 °C until use.
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