Tanca, Alessandro and Pagnozzi, Daniela and Falchi, Giovanni and Biosa, Grazia and Rocca, Stefano and Foddai, Gisella and Uzzau, Sergio and Addis, Maria Filippa (2011) Impact of fixation time on GeLC–MS/MS proteomic profiling of formalin-fixed, paraffin-embedded tissues. Journal of Proteomics, Vol. 74 (7), p. 1015-1021. ISSN 1874-3919. eISSN 1876-7737. Article.
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Formalin-fixed, paraffin-embedded (FFPE) tissue banks represent an invaluable resource for biomarker discovery. Recently, the combination of full-length protein extraction, GeLC–MS/MS analysis, and spectral counting quantification has been successfully applied to mine proteomic information from these tissues. However, several sources of variability affect these samples; among these, the duration of the fixation process is one of the most important and most easily controllable ones. To assess its influence on quality of GeLC–MS/MS data, the impact of fixation time on efficiency of full-length protein extraction efficiency and on quality of label-free quantitative data was evaluated.
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