titoli, abstracts, parole chiave >>>
Effects of postmortem storage temperature on sea bass (Dicentrarchus labrax) muscle protein degradation: analysis by 2-D DIGE and MS

Terova, Genciana and Addis, Maria Filippa and Preziosa, Elena and Pisanu, Salvatore and Pagnozzi, Daniela and Biosa, Grazia and Gornati, Rosalba and Bernardini, Giovanni Battista and Roggio, Tonina and Saroglia, Marco (2011) Effects of postmortem storage temperature on sea bass (Dicentrarchus labrax) muscle protein degradation: analysis by 2-D DIGE and MS. Proteomics, Vol. 11 (14), p. 2901-2910. ISSN 1615-9853. eISSN 1615-9861. Article.

Full text not available from this repository.

DOI: 10.1002/pmic.201100073

Abstract

Storage conditions are known to be important for postmortem deterioration of fish muscle, and temperature is one of the factors with the strongest impact on this process. In order to shed light on the influence of temperature on the status of sea bass (Dicentrarchus labrax) muscle proteins during postmortem storage, a 2-D DIGE and mass spectrometry study was performed on fish kept at either 1 or 18°C for 5 days. As expected, the greatest alterations in sea bass filet protein composition were observed upon postmortem storage at 18°C, with distinct changes appearing in the 2-D protein profile after 5 days of storage at this temperature. In particular, degradation of the myofibrillar protein myosin heavy chain and of the glycolytic enzyme glyceraldehyde-3- phosphate dehydrogenase, among the most abundant muscle proteins, could be clearly observed upon storage at higher temperatures. Although to a lesser extent, however, several proteins were observed to vary in abundance also upon storage for 5 days at 1°C. In particular, one of the most interesting observations was the rapid and significant decrease in the abundance of nucleoside diphosphate kinase B and phosphoglycerate mutase 2, which was observed also at low storage temperatures and appeared to be temperature-independent. The results of this study offer new knowledge on changes occurring in sea bass muscle proteins during postmortem storage at different temperatures and provide indications on protein degradation trends that might be useful for monitoring freshness of fish and quality of storage conditions.

Item Type:Article
ID Code:6188
Status:Published
Refereed:Yes
Uncontrolled Keywords:Animal proteomics, muscle, postmortem, protein, degradation, sea bass, storage temperature
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
Publisher:Wiley
ISSN:1615-9853
eISSN:1615-9861
Deposited On:22 May 2012 17:02

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page