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Immunohistochemical localisation and molecular expression of the steroidogenic enzyme cytochrome P450 17α-hydroxylase ⁄C(17,20)-lyase in the vestibular nuclei of adult male rats

Manca, Paolo and Mulliri, Gabriele and Burrai, Giovanni P. and Pirino, Salvatore and Becciu, Chiara Ombretta (2011) Immunohistochemical localisation and molecular expression of the steroidogenic enzyme cytochrome P450 17α-hydroxylase ⁄C(17,20)-lyase in the vestibular nuclei of adult male rats. Journal of Neuroendocrinology, Vol. 23 (5), p. 444-449. ISSN 0953-8194. eISSN 1365-2826. Article.

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DOI: 10.1111/j.1365-2826.2011.02122.x

Abstract

Many biologically active neurosteroids, including dehydroepiandrosterone (DHEA), are synthesised in the brain. DHEA is a potent endogenous modulator of several neuronal functions, and alterations of DHEA are correlated with various neurobiological deficits. The cytochrome P450 17α-hydroxylase ⁄ C(17,20)-lyase (P450C17) plays a pivotal role in the synthesis of DHEA from pregnenolone and progesterone. We investigated the immunohistochemical localisation and molecular expression of P450C17 in the superior, lateral, medial and inferior vestibular nuclei (VCN) of adult male rats by western blotting and indirect immunofluorescence analysis. Immunoreactive P450C17 was widely distributed in all VCN and the expression of P450C17 was confirmed by western blot analysis. The present study demonstrates, for the first time, the presence and anatomical distribution of P450C17 in the VCN. Given that neurosteroids can modulate neuronal activities in the medial vestibular nucleus, DHEA synthesised in the VCN may play an important role in the control of specific activities at this level.

Item Type:Article
ID Code:6186
Status:Published
Refereed:Yes
Uncontrolled Keywords:P450C17 cytochrome, dehydroepiandrosterone, vestibular nuclei
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Blackwell / Wiley
ISSN:0953-8194
eISSN:1365-2826
Copyright Holders:© 2011 The Authors. Journal of Neuroendocrinology © 2011 Blackwell Publishing Ltd
Deposited On:24 Jun 2011 10:22

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