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Field-amplified online sample stacking capillary electrophoresis UV detection for plasma malondialdehyde measurement

Zinellu, Angelo and Sotgia, Salvatore and Deiana, Luca and Carru, Ciriaco (2011) Field-amplified online sample stacking capillary electrophoresis UV detection for plasma malondialdehyde measurement. Electrophoresis (in press). ISSN 0173-0835. eISSN 1522-2683. Article.

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DOI: 10.1002/elps.201100056

Abstract

Malondialdehyde (MDA) determination is the most widely used method for monitoring lipid peroxidation. Here, we describe an easy field-amplified sample injection (FASI) CE method with UV detection for the detection of free plasma MDA. MDA was detected within 8 min by using 200 mmol/L Tris phosphate pH 5.0 as running buffer. Plasma samples treated with ACN for protein elimination were directly injected on capillary without complex cleanup and/or sample derivatization procedures. Using electrokinetic injection, the detection limit in real sample was 3 nmol/L, thus improving of about 100-fold the LOD of the previous described methods based on CE. Precision tests indicate a good repeatability of our method both for migration times (CV=1.11%) and for areas (CV=2.05%). Moreover, a good reproducibility of intra- and inter-assay tests was obtained (CV=2.55% and CV=5.14%, respectively). Suitability of the method was tested by measuring MDA levels in 44 healthy volunteers.

Item Type:Article
ID Code:6177
Status:Published
Refereed:Yes
Uncontrolled Keywords:CE, field-amplified sample injection, malondialdehyde
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
Publisher: Wiley - VCH Verlag GmbH & Co. KGaA
ISSN:0173-0835
eISSN:1522-2683
Copyright Holders:© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Deposited On:23 Jun 2011 16:38

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