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Synchrotron-based X-ray fluorescence, imaging and elemental mapping from biological samples

Rao, Donepudi Venkateswara and Swapna, Medasani and Cesareo, Roberto and Brunetti, Antonio and Akatsuka, Takao and Yuasa, Tetsuya and Takeda, Tohoru and Gigante, Giovanni Ettore (2011) Synchrotron-based X-ray fluorescence, imaging and elemental mapping from biological samples. Pramana, Vol. 76 (2), p. 261-269. ISSN 0304-4289. eISSN 0973-7111. Article.

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DOI: 10.1007/s12043-011-0031-5


The present study utilized the new hard X-ray microspectroscopy beamline facility, X27A, available at NSLS, BNL, USA, for elemental mapping. This facility provided the primary beam in a small spot of the order of ∼10 μm, for focussing. With this spatial resolution and high flux throughput, the synchrotron-based X-ray fluorescent intensities for Mn, Fe, Zn, Cr, Ti and Cu were measured using a liquid-nitrogen-cooled 13-element energy-dispersive high-purity germanium detector. The sample is scanned in a ‘step-and-repeat’ mode for fast elemental mapping measurements and generated elemental maps at 8, 10 and 12 keV, from a small animal shell (snail). The accumulated trace elements, from these biological samples, in small areas have been identified. Analysis of the small areas will be better suited to establish the physiology of metals in specific structures like small animal shell and the distribution of other elements.

Item Type:Article
ID Code:6159
Uncontrolled Keywords:X-ray fluorescence, synchrotron-based excitation, imaging, elemental mapping, biological samples
Subjects:Area 02 - Scienze fisiche > FIS/07 Fisica applicata (a beni culturali, ambientali, biologia e medicina)
Divisions:001 Università di Sassari > 03 Istituti > Matematica e fisica
Publisher:Springer India, in co-publication with Indian Academy of Sciences
Copyright Holders:© Indian Academy of Sciences
Additional Information:From the issue entitled "Proceedings of the National Conference on X-ray fluorescence", held at Saha institute of nuclear physics, Kolkata (India) from January 12 to 15, 2010
Deposited On:13 Jun 2011 17:23

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