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Field-amplified sample injection combined with pressure-assisted capillary electrophoresis UV detection for the simultaneous analysis of allantoin, uric acid, and malondialdehyde in human plasma

Zinellu, Angelo and Sotgia, Salvatore and Deiana, Luca and Carru, Ciriaco (2011) Field-amplified sample injection combined with pressure-assisted capillary electrophoresis UV detection for the simultaneous analysis of allantoin, uric acid, and malondialdehyde in human plasma. Analytical and Bioanalytical Chemistry, Vol. 399 (8), p. 2855-2861. ISSN 1618-2642. eISSN 1618-2650. Article.

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DOI: 10.1007/s00216-010-4648-x

Abstract

The allantoin/uric acid (All/UA) ratio and malondialdehyde (MDA) plasma levels have been proposed as important markers for monitoring oxidation triggered by the action of free radicals (FR). Here, we describe an easy field amplified sample injection capillary electrophoresis method with UV detection for the separation and quantification of All, UA, and free MDA in human plasma. The plasma samples were simply filtered through centrifugation membrane tubes for protein elimination and directly injected on a capillary without complex cleanup and/or sample derivatization procedures. The use of a run buffer composed of 300 mmol/L sodium borate at pH 10 with 50 mmol/L of N-methyl-Dglucamine and an overimposed pressure/voltage of 0.1 psi during the electrophoretic run allows basline resolution of the analytes within 17 min. The electrokinetic injection allows a detection limit of 15 nmol/L for All, 20 nmol/L for UA and 10 nmol/L for MDA in a plasma sample, thus significantly improving the LOD of previous described methods based on capillary electrophoresis. Precision tests indicate a good repeatability of our method both for migration times (CV=1.85%) and areas (CV=2.87%). Moreover, a good reproducibility of intra- and inter-assay tests was obtained (CV=4.63%and CV=6.59%respectively). The suitability of the method was tested by measuring analyte levels in 40 healthy volunteers.

Item Type:Article
ID Code:6121
Status:Published
Refereed:Yes
Uncontrolled Keywords:Capillary electrophoresis, FASI, allantoin, uric acid, malondialdehyde, oxidative stress
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
Publisher:Springer Berlin / Heidelberg
ISSN:1618-2642
eISSN:1618-2650
Copyright Holders:© Springer-Verlag 2011
Deposited On:08 Jun 2011 15:48

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