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Quantification of neurotransmitter amino acids by capillary electrophoresis laser-induced fluorescence detection in biological fluids

Zinellu, Angelo and Sotgia, Salvatore and Pisanu, Elisabetta and Scanu, Bastianina and Sanna, Manuela and Usai, Maria Franca and Chessa, Roberto and Deiana, Luca and Carru, Ciriaco (2010) Quantification of neurotransmitter amino acids by capillary electrophoresis laser-induced fluorescence detection in biological fluids. Analytical and Bioanalytical Chemistry, Vol. 398 (5), p. 1973-1978. ISSN 1618-2642. eISSN 1618-2650. Article.

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DOI: 10.1007/s00216-010-4134-5

Abstract

The role of neurotransmitter amino acids (NAAs) in the functioning of the nervous system has been the focus of increasingly intense research over the past several years. Among the various amino acids that have important roles as neurotransmitters, there are alanine (Ala), glutamic acid (Glu), aspartic acid (Asp), serine (Ser), taurine (Tau) and glycine (Gly). NAAs are present in plasma, cells and—at trace levels—in all biological fluids, but complex components in biological matrices make it difficult to determine them in biological samples. We describe a new capillary electrophoresis (CE) method with laser-induced fluorescence detection by which analytes are resolved in less than 12 minutes in a 18 mmol/L phosphate run buffer at pH 11.6. The use of elevated temperatures during sample derivatization leads to a drastic reduction in the reaction time, down to 20 min, compared to the 6–14 h usually described for reactions between FITC and amino acids at room temperature. In order to demonstrate its wide range of applications, the method was applied to the analysis of NAA in human plasma and in other sample types, such as red blood cells, urine, cultured cells, cerebrospinal fluid, saliva and vitreous humor, thus avoiding the typical limitations of other methods, which are normally suitable for use with only one or two matrix types.

Item Type:Article
ID Code:6116
Status:Published
Refereed:Yes
Uncontrolled Keywords:Amino acids, capillary electrophoresis, neurotransmitters
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Scienze botaniche, ecologiche e geologiche
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
001 Università di Sassari > 01 Dipartimenti > Zoologia e genetica evoluzionistica
Publisher:Springer Berlin / Heidelberg
ISSN:1618-2642
eISSN:1618-2650
Copyright Holders:© Springer-Verlag 2010
Deposited On:08 Jun 2011 13:11

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